Amphibiocystidium and Amphibiothecum are single-celled parasitic organisms belonging to a group of organisms called dermocystid protozoa that can only be differentiated using molecular genetic methods.

Species affected

Diseases caused by Amphibiocystidium and Amphibiothecum parasites have been reported in a range of amphibians, including frogs, toads, newts and salamanders. Evidence suggests that some amphibian species may be more susceptible to infection than others, but all amphibians should be considered at risk.

Signs of disease

Infection with dermocystid parasites can cause a range of disease outcomes, from mild disease and recovery to death. Infection commonly results in the development of nodular skin lesions that are visible to the naked eye. These vary from small blisters to multiple nodules and large tumour-like lesions, which can become red and ulcerated. The skin lesions can occur anywhere on the body, including the head, tail and legs (see Figures 1 and 2). Sometimes, the lesions caused by Amphibiocystidium are clustered around the vent or cloaca, whereas the ones caused by Amphibiothecum tend to be observed on the animal’s back. Additionally, these dermocystid parasites can infect the liver, in which they form tumour-like lesions of varying sizes.

Figure 1. Alpine newt (Ichthyosaura alpestris) infected with Amphibiocystidium. Long black arrows – ulcerative lesions on back. Short white arrows – coalescent blisters at base of tail. Photo credit: Zoological Society of London.

Figure 2. Palmate newt (Lissotriton helveticus) infected with Amphibiocystidium. Long black arrows – single blisters on body and tail. Short white arrows – coalescent blisters on body. Photo credit: Shaun Denney.

Disease transmission

The life-cycles and means of transmission of these dermocystid parasites are unknown.


Disease consistent with Amphibiocystidium infection was first described in Europe at the beginning of the 20th Century in frogs and newts and has since also been found in North America. There has been an increase in the number of reports of amphibians infected with either of the dermocystid parasites in recent years in Great Britain and elsewhere in Europe; however, this might be due to the large increase in the number of people studying amphibian diseases.
In Great Britain, dermocystid parasitic diseases are suspected to be present in both native and introduced species of newts in multiple sites across the country and their distribution and impact are currently under investigation.

Risk to human health

Amphibiocystidium and Amphibiothecum dermocystid parasites are only known to infect amphibians. There is no known risk to human health.

Risk to domestic animal health

Dermocystid parasites are known to infect various species of amphibian. As such, pet amphibians should be regarded as being susceptible to infection.


A tentative diagnosis of dermocystid parasitic disease can often be made based on the appearance of the lesions found in affected amphibians. However, a definitive diagnosis and differentiation between Amphibiocystidium and Amphibiothecum infection can only be made using specialist laboratory tests.
If you wish to report finding a dead amphibian, or signs of disease in amphibians, please visit Alternatively, if you have further queries or have no internet access, please call the Garden Wildlife Health vets on 0207 449 6685.

Control and prevention

There are no known effective treatments for dermocystid parasitic diseases. The movement of infected animals should be avoided to help prevent the unintentional spread of the parasite to new areas.

Further information

More advice on amphibians in your garden can be found on the Garden Wildlife Health website

Scientific publications

Pascolini, R., Daszak, P., Cunningham, A.A., Tei, S., Vagnetti, D., Bucci, S., Fagotti, A. and Di Rosa, I. (2003) Parasitism by Dermocystidium ranae in a population of Rana esculenta complex in Central Italy and description of Amphibiocystidium n. gen. Diseases of Aquatic Organisms 56(1): 65-74. doi: 10.3354/dao056065.
Raffel, T. R., Bommarito, T., Barry, D.S., Witiak, S.M., and Shackelton, L.A. (2008) Widespread infection of the Eastern red-spotted newt (Notophthalmus viridescens) by a new species of Amphibiocystidium, a genus of fungus-like mesomycetozoan parasites not previously reported in North America. Parasitology 135(2): 203–215. doi:10.1017/S0031182007003708.
Duffus, A.L.J. and Cunningham, A.A. (2010) Major disease threats to European amphibians. The Herpetological Journal 20(3): 117–127.
González-Hernández, M., Denoël, M., Duffus, A.J.L., Garner, T.W.J., Cunningham, A.A. and Acevedo-Whitehouse, K. (2010) Dermocystid infection and associated skin lesions in free-living palmate newts (Lissotriton helveticus) from Southern France. Parasitology International 59(3): 344–350. doi:10.1016/j.parint.2010.04.006.
Fiegna, C., Clarke, C.L., Shaw, D.J., Baily, J.L., Clare, F.C., Gray, A., Garner, T.W.J. and Meredith, A.L. (2017) Pathological and phylogenetic characterization of Amphibiothecum sp. infection in an isolated amphibian (Lissotriton helveticus) population on the island of Rum (Scotland). Parasitology 144(4): 484-496.


Current funding for the GWH comes in part from Defra, the Welsh Government and the Animal and Plant Agency (APHA) Diseases of Wildlife Scheme (DoWS); and from the Esmée Fairbairn Foundation and the Universities Federation for Animal Welfare.


This fact sheet was produced by Garden Wildlife Health (GWH) for information purposes only. The GWH will not be liable for any loss, damage, cost or expense incurred in or arising by reason of any person relying on information in this fact sheet.

Date of factsheet update:

September 2017